Urinary excretion of diazepam metabolites in healthy volunteers and drug users

Urinary excretion profiles of diazepam metabolites were investigated. The s ubjects were healthy volunteers receiving one single 10-mg dose of diazepam or drug abusers starting a prison sentence. Urinary excretion of metabolit es was analysed by immunological screening, liquid chromatography and gas c hromatography-mass spectrometry. Relating the metabolite concentration to c reatinine concentration in the specimens decreased sample-to-sample variati ons. In some cases such correction could protect a subject from erroneous a ccusations of a new intake.