Genetic diversity of rhizobia from natural populations varies with the soil dilution sampled

Authors
Bala, A Murphy, P Giller, KE
Citation
A. Bala et al., Genetic diversity of rhizobia from natural populations varies with the soil dilution sampled, SOIL BIOL B, 33(6), 2001, pp. 841-843
Citations number
18
Categorie Soggetti
Environment/Ecology
Journal title
SOIL BIOLOGY & BIOCHEMISTRY
ISSN journal
00380717 → ACNP
Volume
33
Issue
6
Year of publication
2001
Pages
841 - 843
Database
ISI
SICI code
0038-0717(200105)33:6<841:GDORFN>2.0.ZU;2-E
Abstract
Rhizobia were isolated from root nodules of three tree legumes inoculated w ith dilution series of two tropical soils. The diversity of the isolates ob tained from different soil dilutions was assessed by PCR-RFLP analysis of t he internally transcribed spacer (ITS) region between the 165 and 23S rRNA genes. In both soils there was a strong effect of dilution on the genetic d iversity of the populations with apparent diversity decreasing with increas ing dilution. (C) 2001 Elsevier Science Ltd. All rights reserved.