Mycobactin J 1 is a commercially available siderophore isolated from Mycoba
cterium avium subsp, paratuberculosis. There are discrepancies between prev
ious reports of its structure and none have addressed its absolute configur
ation. We report here the complete structure and stereochemistry of mycobac
tin J, along with methodology to enable the determination of the absolute c
onfiguration of other mycobactins on a small scale. (C) 2001 Elsevier Scien
ce Ltd. All rights reserved.