In situ F-19 NMR spectroscopy study of enzymatic transglycosylation reactions using alpha-D-aldohexopyranosyl fluorides as donors and accepters

The use of F-19 NMR spectroscopy to study the kinetics of the self-condensa tion reaction of alpha -D-aldohexopyranosyl fluorides catalysed by alpha -g lycosidases is described. The corresponding fluorinated disaccharides thus: synthesised present separate individual fluorine resonances allowing the i ntegration of each species. This method looks particularly useful to help i n the choice of donor in enzymatic transglycosylation reactions since the s elf-condensation reaction always remains in competition with the condensati on reaction (reaction of the donor with accepters other than itself). (C) 2 001 Elsevier Science Ltd. All rights reserved.