B. Bolgiano et al., Effect of physico-chemical modification on the immunogenicity of Haemophilus influenzae type b oligosaccharide-CRM197 conjugate vaccines, VACCINE, 19(23-24), 2001, pp. 3189-3200
Haemophilus influenzae type b (Hib) poly-ribosyl-ribityl phosphate (PRP) ol
igosaccharide-CRM197 conjugate vaccines from two different manufacturers (H
ib A and Hib B) were subjected to adverse storage conditions and used to es
tablish correlates between physico-chemical characteristics and immunogenic
ity. There were manufacturer-specific differences in the effect of freezing
or freeze-thawing on the carrier protein conformation and the anti-CRM197
or anti-PRP IgG response in rabbits whereas both conjugates showed similar
stability when stored at elevated temperatures. Both oligosaccharide-CRM197
conjugate vaccines formed apparent aggregates' of cion-specifically associ
ated higher molecular weight material when subjected to elevated temperatur
es or repeated freeze-thawing. Following subcutaneous injection of samples
into CBA mice and New Zealand White rabbits, the amount of IgG raised again
st CRM197 was significantly lower for samples incubated at 37 or 55 degrees
C compared with those kept at 4 degreesC, consistent with the less well-fol
ded conformation of the carrier protein observed at elevated temperatures.
Moreover, there was a parallel reduction in the amount of IgG raised agains
t PRP and the level of bactericidal antibodies induced by vaccines A and B
stored at 55 degreesC consistent with the observed depolymerisation of the
oligosaccharide chains. Carrier protein conformational changes resulting fr
om storage under adverse conditions did not affect the immunogenicity to Hi
b PRP in laboratory animals unless associated with loss of bound saccharide
presumably because the carrier protein retains continuous T-H cell epitope
s which are unaffected by conformational changes. Crown Copyright Q 2001 Pu
blished by Elsevier Science Ltd. All rights reserved.