INTERFERON MEDIATED PHOSPHATIDYLINOSITOL UPTAKE AND PROCESSING IN NUCLEI ISOLATED FROM BURKITT-LYMPHOMA CELLS

The kinetic analysis of exogenous [H-3]phosphatidylinositol (PI) uptak e and processing by nuclei isolated from Daudi lymphoma cells upon int erferon alpha treatment has been performed. Results have disclosed tha t, with respect to controls, interferon induces an evident stimulation of label incorporation into nuclei. The incorporated [H-3] PI has bee n found for phosphorylation and hydrolytic cleavage, indicating that t he intranuclear transduction system activated by interferon at plasma membrane level, might involve the PI cycle as a possible route of intr acellular signalling.