CENTA as a chromogenic substrate for studying beta-lactamases

Authors
Bebrone, C Moali, C Mahy, F Rival, S Docquier, JD Rossolini, GM Fastrez, J Pratt, RF Frere, JM Galleni, M
Citation
C. Bebrone et al., CENTA as a chromogenic substrate for studying beta-lactamases, ANTIM AG CH, 45(6), 2001, pp. 1868-1871
Citations number
23
Categorie Soggetti
Microbiology
Journal title
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
ISSN journal
00664804 → ACNP
Volume
45
Issue
6
Year of publication
2001
Pages
1868 - 1871
Database
ISI
SICI code
0066-4804(200106)45:6<1868:CAACSF>2.0.ZU;2-A
Abstract
CENTA, a chromogenic cephalosporin, is readily hydrolyzed by B-lactamases o f all classes except for the Aeromonas hydrophila metalloenzyme. Although i t cannot practically be used for the detection of beta -lactamase-producing strains on agar plates, it should be quite useful for kinetic studies and the detection of the enzymes in crude extracts and chromatographic fraction s.