Kanamycin susceptibility testing of Mycobacterium tuberculosis using mycobacterium growth indicator tube and a colorimetric method

Authors
Bastian, I Rigouts, L Palomino, JC Portaels, F
Citation
I. Bastian et al., Kanamycin susceptibility testing of Mycobacterium tuberculosis using mycobacterium growth indicator tube and a colorimetric method, ANTIM AG CH, 45(6), 2001, pp. 1934-1936
Citations number
21
Categorie Soggetti
Microbiology
Journal title
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
ISSN journal
00664804 → ACNP
Volume
45
Issue
6
Year of publication
2001
Pages
1934 - 1936
Database
ISI
SICI code
0066-4804(200106)45:6<1934:KSTOMT>2.0.ZU;2-H
Abstract
Two novel systems were evaluated for performing indirect kanamycin suscepti bility tests on 72 strains of Mycobacterium tuberculosis. The microplate Al amar blue colorimetric method (breakpoint, 2.5 mug/ml) and the Mycobacteriu m Growth Indicator Tube (MGIT) system (breakpoint, 5.0 mug/ml) both produce d 98.6% agreement when compared with the conventional proportion method per formed on 7H10 agar using 5.0 pg of kanamycin/ml. Both systems provided res ults within an average of 1 week.