Bw. Faber et al., Constitutive and inducible hydroxylase activities involved in the degradation of naphthalene by Cunninghamella elegans, APPL MICR B, 55(4), 2001, pp. 486-491
The non-ligninolytic fungus Cunninghamella elegans was investigated for its
ability to produce naphthalene hydroxylase (NAH) and naphthol hydroxylase
(NOH) activities under various conditions. When the organism was cultivated
on a rich growth medium, the mycelia exhibited significant constitutive NA
H activity in the late exponential growth phase, but not in the early-expon
ential-growth-phase. On incubating the early-exponential-growth-phase mycel
ia with naphthalene, NAH activity was increased five-fold; however, this in
crease did not occur in the presence of the protein synthesis inhibitor cyc
loheximide. Since incubation of the late-phase mycelia with naphthalene did
not lead to a higher degradation rate of naphthalene, mycelia in this phys
iological state have apparently lost the ability to induce synthesis of the
enzyme exhibiting NAH activity. This is not due to an overall inability to
perform de novo protein synthesis, since NOH activity, non-constitutive at
all growth phases, could be induced by incubating late-phase mycelia with
naphthalene. Whether inducible and constitutive NAH activity originate from
one and the same enzyme remains to be elucidated. It is suggested that nap
hthalene oxidizing enzyme(s) may also oxidize pyrene, but not anthracene or
benzo[a]pyrene, although the latter are degradable by C. elegnns.