Partially deacetylated chitins with different degrees of deacetylation (DD)
were prepared by alkaline treatment under homogeneous conditions, and the
effect of DD on their solubility was discussed in terms of crystal structur
e and mode of hydrogen bonding. With an increase in the treatment time, the
DD of chitin increased proportionally. The chitin became soluble in dilute
acetic acid at the DD of ca. 28% or over and soluble in water at the DD of
ca. 49%. The solubility of the partially deacetylated chitins had a close
relationship with their crystal structure, crystallinity, and crystal imper
fection as well as the glucosamine content. The wide-angle X-ray diffractom
etry (WAXD) revealed that the chitin with ca. 28% DD retained the crystal s
tructure of alpha -chitin with significantly reduced crystallinity and perf
ection of the crystallites. The water-soluble chitin of ca. 49% DD had a ne
w crystal structure similar to that of beta -chitin rather than either alph
a -chitin or chitosan, suggesting that the homogeneous deacetylation transf
ormed the crystal structure of chitin from the alpha to the beta form. Some
hydrogen bonds existing in raw alpha -chitin were found to be missing at a
DD of ca. 49%.