Solid state NMR for determination of degree of acetylation of chitin and chitosan

The degree of acetylation (DA) of various samples has been evaluated by H-1 liquid-state NMR and C-13 and N-15 CP-MAS solid-state NMR over the whole r ange of DA. A good agreement has been found for all the experiments. The C- 13 and N-15 CP-MAS experiments have permitted the evaluation of the chitin DA and content in the structural polysaccharides in a fungus named Aspergil lus niger. The fungus structural carbohydrates mainly consist in pure chiti n associated with glucans. Comparison of the C-13 CP-MAS spectra with stand ard (1-->3)-beta -D-glucans strongly suggests that chitin and glucans are l inked via covalent bonds.