Self-assembly of an oligodeoxyribonucleotide harboring the estrogen response element in the presence of polyamines: Ionic, structural, and DNA sequence specificity effects

Estrogenic regulation of gene expression is mediated by the binding of the hormone to its specific receptor, estrogen receptor (ER), which undergoes s tructural and conformational alterations to recognize specific DNA sequence s, estrogen response elements (ERE), in responsive genes to trigger a serie s of events culminating in the transcription of these genes. Polyamines are ubiquitous cellular cations that are important for cell growth and differe ntiation, and have been shown to participate in estrogenic regulation of ge ne expression. Polyamine-mediated DNA condensation/aggregation has been stu died to understand the ionic and structural requirements for the compaction of DNA. DNA condensation/decondensation may also play a role in transcript ion and replication. We studied the aggregation of a 38-mer oligonucleotide duplex (ODN) in the presence of natural and synthetic polyamines under dif ferent ionic conditions (NaCl, KCl, and K glutamate). Our results showed th at an ODN harboring the consensus ERE (ODN1) was 2-fold more susceptible to precipitation by spermine compared to ODN2 containing scrambled sequences, or a mutant ODN (ODN3). The nature of the monovalent cations (Na+ vs K+), and anions (Cl- vs glutamate) also played an important role in the efficacy of a polyamine to precipitate ODNs: potassium glutamate being the least ef fective in suppressing the ability of spermine to precipitate ODNs. The con centration of polyamines required for precipitating the ODNs increased with monovalent ion concentration in the buffer. With ODN1, a plot of log[sperm ine(4+)] at the 50% precipitation concentrations against fog[Na+/K+] yielde d a straight line, with a slope of 1.8 +/- 0.18, a value comparable to that predicted by the counterion condensation theory (1.85). We also observed s ignificant structural specificity effects of spermine and its analogues [NH 2(CH2)(3)NH(CH2)(n)NH(CH2)(3)NH2, where n = 2-9; n = 4 for spermine] on agg regating the ODN1. These results demonstrate DNA sequence and polyamine str uctural specificity effects on the aggregation of ODNs, and suggest that th e gene regulatory function of ERE may be linked to its ability to undergo f acile condensation/ decondensation in the presence of biological cations, s uch as polyamines.