Novel method using phytase for separating soybean beta-conglycinin and glycinin

Authors
Saito, T Kohno, M Tsumura, K Kugimiya, W Kito, M
Citation
T. Saito et al., Novel method using phytase for separating soybean beta-conglycinin and glycinin, BIOS BIOT B, 65(4), 2001, pp. 884-887
Citations number
19
Categorie Soggetti
Agricultural Chemistry","Biochemistry & Biophysics
Journal title
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
ISSN journal
09168451 → ACNP
Volume
65
Issue
4
Year of publication
2001
Pages
884 - 887
Database
ISI
SICI code
0916-8451(200104)65:4<884:NMUPFS>2.0.ZU;2-9
Abstract
A novel method for separating sogbean beta -conglycinin and glycinin from d efatted soymilk by a phytase treatment was developed. Phytase was added to defatted soymilk (1000FYT/100 g of protein) at pH 6.0, and the mixture incu bated for 1 h at 40 degreesC, This procedure separated beta -conglycinin an d glycinin without seeding a reducing agent or cooling into the soluble and insoluble fractions, respectively. Simultaneously most of the phytate in b oth proteins was removed.