If. Palm et al., The stimulatory effect of vasopressin on the luteinizing hormone surge in ovariectomized, estradiol-treated rats is time-dependent, BRAIN RES, 901(1-2), 2001, pp. 109-116
The luteinizing hormone surge in the female rat is not only induced by the
positive feedback of estradiol, but also by circadian signals originating i
n the suprachiasmatic nucleus (SCN). In a previous study we showed that adm
inistration of vasopressin. an SCN transmitter present in preoptic projecti
ons, induced an LH surge in animals bearing complete lesions of the SCN. Th
is strongly suggests vasopressin as a stimulatory circadian signal for the
timing of the LH surge. In the present study we investigated during which t
ime window vasopressin may act in the medial preoptic area to stimulate LH
secretion in SCN-intact female rats. Vasopressin or a specific Via receptor
antagonist was administered into the MPO by a reverse microdialysis techni
que during different time windows, and plasma LH concentrations were measur
ed. Vasopressin stimulated the LH surge in 30% of the animals, when adminis
tered during the second half of the light period, but during the first half
of the light period no effects were observed. Administration of the Via re
ceptor antagonist, however, did not affect the LH surge. These data confirm
our previous results that vasopressin is a stimulatory factor for the LH s
urge also in SCN-intact animals, and indicate that a certain time window is
available for such stimulation. We hypothesize that vasopressin in the SCN
-intact animal may act as a circadian signal during a specific time window
to induce the LH surge. The time window is the result of other SCN regulato
ry systems that are involved in the preparation of the LH surge. (C) 2001 E
lsevier Science B.V. All rights reserved.