Detection of spores of Nucleospora salmonis in frozen tissue material fromAtlantic salmon

Authors
Guzman, O Enriquez, R Schottelius, J
Citation
O. Guzman et al., Detection of spores of Nucleospora salmonis in frozen tissue material fromAtlantic salmon, B EUR ASS F, 21(2), 2001, pp. 81-83
Citations number
5
Categorie Soggetti
Aquatic Sciences
Journal title
BULLETIN OF THE EUROPEAN ASSOCIATION OF FISH PATHOLOGISTS
ISSN journal
01080288 → ACNP
Volume
21
Issue
2
Year of publication
2001
Pages
81 - 83
Database
ISI
SICI code
0108-0288(2001)21:2<81:DOSONS>2.0.ZU;2-E
Abstract
In contrast to the detection of spores of Nucleospora salmonis in fresh tis sue material of Salmo salar by Gram staining, it was not possible to stain these spores in the same material when it has been kept frozen and then def rosted at 4 degreesC over several hours. The spores could only be made visi ble by incubation of the imprints of the defrosted and then sectioned kidne y and spleen material in calcofluor and by a double staining employing Gram staining with subsequent incubation in Calcofluor.