On the biosynthesis and characterization of cellulasic enzymes. II. Cellulase production enhancement in different biosynthesis conditions

A possible maximization of the biosynthesis process of cellulases involves the establishment of some peculiar conditions of cultivation, so that the b iological potential of the microorganism should be most propitiously utiliz ed. The experiments carried out using two fungal strains, Phanerochaete chr ysosporium and Chaetomium elatum have shown a different behaviour to the va riation of some factors of influence, such as: the cellulosic inducer, the biological material for inoculation, the type of medium, the hydrodynamic c onditions, pH and temperature of cultivation. The enzymatic activities, the extracellular protein content, the pH variation and the behaviour of biosy nthetized cellulasic complex in hydrolysis reactions have been also analyze d in order to establish the optimum conditions for which the whole complex or only one of its components may be produced.