Improvement of FISH mapping resolution on combed DNA molecules by iterative constrained deconvolution: a quantitative study

Authors
Monier, K Heliot, L Rougeulle, C Heard, E Robert-Nicoud, M Vourc'h, C Bensimon, A Usson, Y
Citation
K. Monier et al., Improvement of FISH mapping resolution on combed DNA molecules by iterative constrained deconvolution: a quantitative study, CYTOG C GEN, 92(1-2), 2001, pp. 59-62
Citations number
9
Categorie Soggetti
Molecular Biology & Genetics
Journal title
CYTOGENETICS AND CELL GENETICS
ISSN journal
03010171 → ACNP
Volume
92
Issue
1-2
Year of publication
2001
Pages
59 - 62
Database
ISI
SICI code
0301-0171(2001)92:1-2<59:IOFMRO>2.0.ZU;2-I
Abstract
Image restoration approaches, such as digital deconvolution, are becoming w idely used for improving the quality of microscopic images. However, no qua ntification of the gain in resolution of fluorescence images is available. We show that, after iterative constrained deconvolution, fluorescent cosmid signals appear to be 25 % smaller, and 1.2-kb fragment signals on combed m olecules faithfully display the expected length. Copyright (C) 2001 S. Karg er AG, Basel.