Induction and characterization of hypoxanthine-phosphoribosyltransferase (Hprt(-)) deficient cell lines of Akodon cursor (Rodentia, Sigmodontinae)

Authors
Bonvicino, CR Moreira, MAM Arcuri, RA Seuanez, HN
Citation
Cr. Bonvicino et al., Induction and characterization of hypoxanthine-phosphoribosyltransferase (Hprt(-)) deficient cell lines of Akodon cursor (Rodentia, Sigmodontinae), CYTOG C GEN, 92(1-2), 2001, pp. 153-156
Citations number
7
Categorie Soggetti
Molecular Biology & Genetics
Journal title
CYTOGENETICS AND CELL GENETICS
ISSN journal
03010171 → ACNP
Volume
92
Issue
1-2
Year of publication
2001
Pages
153 - 156
Database
ISI
SICI code
0301-0171(2001)92:1-2<153:IACOH(>2.0.ZU;2-G
Abstract
Hypoxanthine-phosphoribosyltransferase negative (Hprt(-)) cell lines derive d from an Akodon cursor liposarcoma were obtained by induced mutagenesis. A ll but one Hprt(-) cell line lacked Hprt mRNA transcripts while one (AKO 3) coded for a truncated protein. Cell fusion and karyotypic analyses showed that one cell line (AKO 1-15) could be successfully used for constructing h ybrid panels and allow for a clear identification of human chromosomes in h ybrid cells. Copyright (C) 2001 S. Karger AG, Basel.