Development of the rat efferent vestibular system on the ground and in microgravity

We investigated whether plastic changes occurred in the organization of the vestibular efferent network in the rat utricle during a 17-day episode of microgravity, from postnatal (PN) day 8 to PN23, and on return to earth on PN25. We also determined the normal pattern of efferent development from bi rth to PN25. Immunofluorescence experiments were performed with a specific biochemical marker of the efferent system, the calcitonin gene-related pept ide (CGRP), and vibratome sections of the utricles were analyzed by laser s canning confocal microscopy. At birth, a few efferent fibers were detected beneath the sensory epithelium. These then massively invaded the epithelium between PN2 and PN4. At the time of launch, PN8, most fiber paths in the u tricular epithelium, after following transient courses (towards the epithel ial surface for example) returned to the base and were stabilized in the lo wer part of the epithelium, in which they established synaptic contacts wit h sensory cells, except at a few immature locations. The main difference be tween this stage ton PN8) and subsequent more mature stages was the lower d ensity of fibers and synapses in the utricle. The maturation of the vestibu lar efferent system was similar in microgravity and on the ground. Thus, ma turation of the efferent system between PN8 and PN23 was not sensitive to a change in gravitational environment. These results suggest that periods of microgravity at earlier stages are required to identify critical periods i n peripheral vestibular system development. (C) 2001 Elsevier Science B.V. All rights reserved.