J. Lom et al., Redescription of Microsporidium takedai (Awakura, 1974) as Kabatana takedai (Awakura, 1974) comb. n., DIS AQU ORG, 44(3), 2001, pp. 223-230
Ultrastructural study of the microsporidian Microsporidium takedai from the
muscles of masu salmon Oncorhynchus masou proved that this species can be
assigned to the genus Kabatana Lom, Dykova and Tonguthai, 2000. The parasit
es develop within disintegrated sarcoplasm without any delimiting boundary
or cyst. Cylindrical multinucleate meronts proliferate by serial constricti
ons into uninucleate stages which repeat the process. Eventually, the uninu
cleate stages transform into uninucleate sporonts, which divide once to pro
duce sporoblasts, thus functioning as sporoblast mother cells. Spores, with
a subterminally located anchoring disc and 3 to 4 turns of the polar tube
coil, average 3.3 by 1.9 mum in size. The exospore is divided into small fi
elds; the endospore frequently makes small invaginations into the spore ins
ide. Phylogenetic analysis using SSU rDNA sequence consistently placed Kaba
tana takedai in a group consisting of Microgemma sp., Spraguea lophii and G
lugea americanus. The K, takedai could easily be separated from the other s
pecies in the same group by 2 inserts in the SSU rDNA sequence.