Construction of a photogalvanic cell using photosynthetic reaction center complex and cytochrome c(2) from Rhodospirillum rubrum

The photosynthetic reaction center complex was immobilized on a p-benzoquin onethiol-modified Au electrode. The potential of the reaction center-immobi lized Au electrode in 0.1 M (M = mol/dm(3)) Tris-HCl buffer (pH 7.5) was sh ifted, upon illumination, to the negative direction, and the value increase d with increasing the intensity of incident light. In addition, the potenti al shift was enhanced by adding cytochrome c(2) to the solution. A photogal vanic cell was constructed only using this electrode, cytochrome c(2), and an ITO electrode with hydrophilic surface, and the cell performance was exa mined. The open-circuit photovoltage and the short-circuit photocurrent wer e about 5 mV and 40 nA, respectively.