Wk. Huh et al., Deficiency of D-erythroascorbic acid attenuates hyphal growth and virulence of Candida albicans, INFEC IMMUN, 69(6), 2001, pp. 3939-3946
In some lower eukaryotes, D-erythroascorbic acid, a five-carbon analog of L
-ascorbic acid, is present instead of L-ascorbic acid. We have cloned ALO1,
the gene encoding D-arabinono-1,4-lactone oxidase, which catalyzes the fin
al step of D-erythroascorbic acid biosynthesis in Candida albicans. The ALO
1 gene contained a continuous open reading frame of 1,671 bp that encodes a
polypeptide consisting of 557 amino acids with a calculated molecular mass
of 63,428 Da. To investigate the functional roles of D-erythroascorbic aci
d in C. albicans, we disrupted or overexpressed the ALO1 gene. In the alo1/
alo1 null mutants, the activity of D-arabinono-1,4-lactone oxidase was comp
letely lost and D-erythroascorbic acid could not be detected. When ALO1 on
a multicopy plasmid was transformed in C. albicans, the enzyme activity and
the intracellular D-erythroascorbic acid level were increased up to 3.4-fo
ld and 4.0-fold, respectively. The alo1/alo1 null mutants of C. albicans sh
owed increased sensitivity towards oxidative stress. Overexpression ofALO1
made the cells more resistant to the same stress. The alo1/alo1 mutants sho
wed defective hyphal growth and attenuated virulence. Taken together, our r
esults suggest that D-erythroascorbic acid functions as an important antiox
idant and can be considered one of the virulence factors enhancing the path
ogenicity of C. albicans.