This article presents the results of a study designed to evaluate the use o
f pathogen surrogates as a tool in treatment optimization and to predict pa
thogen occurrence. A comprehensive matrix was developed to relate water qua
lity and process variables for 24 potable water sources and 12 possible sur
rogates to the target pathogens of Giardia, Cryptosporidium, and enteric vi
ruses. No "ideal" surrogate for predicting occurrence of protozoan pathogen
s or treatment performance was identified because of analytical constraints
and low pathogen occurrence. However, it was concluded that aerobic spores
and particle counts could be used as surrogate measures to evaluate or imp
rove treatment plant performance. Utilities should select not one but a num
ber of surrogates to assess raw water quality and treatment plant performan
ce.