Multiple mobile promoter regions for the rare carbapenem resistance gene of Bacteroides fragilis

Two novel insertion sequences (IS), IS1187 and IS1188, are described upstre am from the carbapenem resistance gene cfiA in strains of Bacteroides fragi lis. Mapping, with the RACE procedure, of transcription start sites of cfiA in these and two other previously reported IS showed that transcription of this rarely encountered gene is initiated close to a variety of B. fragili s consensus promoter sequences, as recently defined (D. P. Bayley, E. R. Ro cha, and C. J. Smith, FEMS Microbiol. Lett. 193:149-154, 2000). In the case s of IS1186 and IS1188, these sequences overlap with putative E sigma (70) promoter sequences, while in IS942 and IS1187 such sequences can be observe d either upstream or downstream of the B. fragilis promoters.