The effect of Celsior solution on 12-hour cardiac preservation in comparison with University of Wisconsin solution

Background Celsior is a new extracellular-type preservation solution which has been developed to act not only as a storage medium but also as a perfus ion fluid during initial donor heart arrest, poststorage graft reimplantati on and early reperfusion. We designed this experimental study to evaluate t he effect of the Celsior solution in comparison with the University of Wisc onsin solution from the viewpoint of energy depletion. Methods. Adult mongrel dogs weighing 9 to 13 kg were divided into two group s. In the UW group (n = 7), a 4 degreesC University of Wisconsin solution w as used for coronary vascular washout and storage following cardiac arrest using a glucose-insulin-potassium solution. In the Celsior group (n = 7), t he Celsior solution was used to obtain cardiac arrest, coronary vascular wa shout and storage. High energy phosphate levels and myocardial pH were meas ured using P-31-nuclear magnetic resonance spectroscopy immediately after p reservation and at 3, 6 and 12 hours after preservation. After 12-hour cold storage, left ventricular free wall tissues were harvested for histologica l examination. Results, High energy phosphate levels and myocardial pH were significantly better preserved in the Celsior group than in the UW group. In the histolog ical findings, glycogen granules were preserved well in. the Celsior group. Conclusions. We conclude from our study that the Celsior solution is compar able to the University of Wisconsin solution for use in 12-hour heart prese rvation in canine models.