Ac. Siani et al., Chemical composition and anti-inflammatory activity of the hydrodistillatefrom Mariscus pedunculatus, J BRAZ CHEM, 12(3), 2001, pp. 354-359
A mixture of the benzofuran remirol and the benzopyran isoevodionol (5.7:1)
was extracted by hydrodistillation from rhizomes of Mariscus pedunculatus.
They were purified by successive thin layer chromatography. Their structur
e elucidation were confirmed by 2D-NMR experiments. The structure of remiro
l was also confirmed by its hydrogenation product. Crude distillate and the
pure compounds were screened for anti-inflammatory activity by the use of
the mouse pleurisy model induced by lipopolysaccharide (LPS, 12.5 ng/ cavit
y). Both the mixture and remirol showed a dose-dependent (100 and 200 mg kg
(-1) p.o.) capacity to inhibit neutrophil and eosinophil accumulation in th
e mouse pleural cavity. In contrast, no anti-inflammatory effect was observ
ed for iso-evodionol.