PREPARATION AND CHARACTERIZATION OF 2 MONOCLONAL-ANTIBODIES AGAINST OXIDATIVELY MODIFIED LOW-DENSITY-LIPOPROTEIN (O-LDL) AND DETECTION OF O-LDL IN SERUM IN PATIENTS WITH CORONARY HEART-DISEASE
M. Zhou et al., PREPARATION AND CHARACTERIZATION OF 2 MONOCLONAL-ANTIBODIES AGAINST OXIDATIVELY MODIFIED LOW-DENSITY-LIPOPROTEIN (O-LDL) AND DETECTION OF O-LDL IN SERUM IN PATIENTS WITH CORONARY HEART-DISEASE, Medical science research, 25(7), 1997, pp. 445-449
There is much evidence that oxidatively modified low-density lipoprote
in (O-LDL) plays an important role in the pathogenesis of atherosclero
sis. To investigate this further and to facilitate early diagnosis of
atherosclerosis, we raised two monoclonal antibodies (mAbs) against O-
LDL. Competitive assay showed that one of these, HOL3, recog nised O-L
DL oxidatively modified for 2, s and 16 h. The other, HOL5, interacted
only with O-LDL oxidatively modified for 8 and 16 h. The immunologica
l reactivities of the mAbs with serum of healthy donors indicated that
the reactivity of HOL3 was stronger than that of HOL5. Compared with
healthy donors, the corresponding optical density (OD) values of O-LDL
in serum of coronary heart disease patients produced by the two mAbs
were all higher, but the degrees of the increase were different. The i
ncrease in percentage of serum O-LDL detected with HOL5 in the CHD pat
ients was 337%, while that with HOL3 was only 37%. Therefore, the mAbs
, especially HOL5, may be useful tools for investigating O-LDL in the
circulation of CHD patients. Because lipid peroxidation and subsequent
modification of LDL also occur in some other diseases, these immunolo
gical reagents could also be useful in studies on their pathogenesis.