A soil pseudomonad capable of metabolizing either alpha- or beta -pinene as
sole carbon and energy source was isolated with an enrichment culture. Cul
ture broth in mineral media gave rise to a pine-like aroma during the initi
al fermentation stage and a fresh rose-like aroma during the late fermentat
ion stage. No growth inhibition was found up to 10% pinenes concentration.
The bioconversion products of cx-pinene were identified as limonene, p-cyme
ne, alpha -terpinolene, camphor, terpinen-4-ol, a-terpineol, endo-borneol,
and p-cymene-8-ol. The bioconversion products of beta -pinene were the same
except for the presence of fenchyl alcohol and the absence of camphor. Mos
t of the accumulated products were p-menthene derivatives such as p-cymene,
limonene, and alpha -terpinolene in both substrates. Total bioconversion w
as 33.5% of alpha -pinene and 58.8% of beta -pinene. (C) 2001 Elsevier Scie
nce Ltd. All rights reserved.