Thiobacillus ferrooxidans and Thiobacillus thiooxidans significantly enhanc
es heazelwoodite (Ni3S2) dissolution. In pure cultures, heazelwoodite was m
ore efficiently leached by T. ferrooxidans in the absence of alternative en
ergy sources and in cultures supplemented with iron(II) and sulphur. In the
other cultures, pure T. thiooxidans cultures reached greater extractions i
n the presence of iron(III). Under all experimental conditions, mixed cultu
res were more efficient than pure cultures. Nickel extraction in mixed cult
ures can be analysed as a cooperation of the mechanisms involved in the pur
e cultures of T. ferrooxidans and T. thiooxidans. (C) 2001 Elsevier Science
Ltd. All rights Reserved.