Polypyrrole electrospotting for the construction of oligonucleotide arrayscompatible with a surface plasmon resonance hybridization detection.

We describe in this paper a new electrospotting process allowing a straight forward functionalization of homogeneous gold surfaces. It is based on a di rect electro-copolymerization of pyrrole and pyrrole linked to an oligonucl eotide on a gold substrate via the use of a moving micro electrochemical ce ll. The polypyrrole spots obtained by this way were characterized in terms of thickness and DNA hybridization efficiency. Results show that either flu orescence or surface plasmon resonance detection of DNA hybridization can b e carried out on these substrates.