Flavonoids, phenolic acids and sesquiterpene lactones were evaluated as qua
litative and quantitative chemosystematic markers in the genus Leontodon by
HPLC-UV and HPLC-MS analyses. One population of Leontodon hispidus L. show
ed a high degree of variation between different plant organs and between di
fferent individuals. Seasonal variation of phenolic acids in the leaves and
of sesquiterpene lactones in the rhizomes was also pronounced. In contrast
. phenolics in the heads were present in nearly constant amounts during the
flowering period. Within species, analyses of samples originating from dif
ferent altitudes (13 samples of L. helveticus from 1600-2950 m, 19 samples
of L. autumnalis from 30-2075 m, and 61 samples of L. hispidus from 40-2550
m) exhibited a significant positive correlation between altitude of the co
llection sites and total content of flavonoids. Unlike flavonoids, the phen
olic acids showed no significant altitudinal variation. The data reveal mor
e pronounced variation between rather than within species.