FOURIER-TRANSFORM INFRARED SPECTROSCOPIC STUDY OF INTACT-CELLS OF THENITROGEN-FIXING BACTERIUM AZOSPIRILLUM-BRASILENSE

The data of Fourier transform infrared (FTIR) spectroscopic measuremen ts performed on intact cells of the soil nitrogen-fixing bacterium Azo spirillum brasilense grown in a standard medium and under the conditio ns of an increased metal uptake are compared and discussed. The struct ural FTIR information obtained is considered together with atomic abso rption spectrometry (AAS) data on the content of metal cations in the bacterial cells. Some methodological aspects concerning preparation of bacterial cell samples for FTIR measurements are also discussed. (C) 1997 Elsevier Science B.V.