EFFECT OF SALT AND ORGANIC-SOLVENTS ON DNA THERMAL-STABILITY AND STRUCTURE

Authors
SOROKIN VA GLADCHENKO GO VALEEV VA SYSA IV PETROVA LG BLAGOI YP
Citation
Va. Sorokin et al., EFFECT OF SALT AND ORGANIC-SOLVENTS ON DNA THERMAL-STABILITY AND STRUCTURE, Journal of molecular structure, 408, 1997, pp. 237-240
Citations number
9
Categorie Soggetti
Chemistry Physical
Journal title
Journal of molecular structure → ACNP
ISSN journal
00222860
Volume
408
Year of publication
1997
Pages
237 - 240
Database
ISI
SICI code
0022-2860(1997)408:<237:EOSAOO>2.0.ZU;2-4
Abstract
At low ionic strength the thermal stability of DNA was observed to be strongly enhanced by the presence of glycerol. The stabilizing effect went down as the Na+ concentration was increased. With high concentrat ions of the ions or glycerol a further increase in the glycerol concen tration suppressed the thermal stability of DNA. The effects observed may be attributed to enhanced Coulombic Na+-DNA interaction as the die lectric constant of the solution decreases. (C) 1997 Elsevier Science B.V.