Single molecule nanomanipulation of biomolecules

Citation
Y. Ishii et al., Single molecule nanomanipulation of biomolecules, TRENDS BIOT, 19(6), 2001, pp. 211-216
Citations number
34
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
TRENDS IN BIOTECHNOLOGY
ISSN journal
01677799 → ACNP
Volume
19
Issue
6
Year of publication
2001
Pages
211 - 216
Database
ISI
SICI code
0167-7799(200106)19:6<211:SMNOB>2.0.ZU;2-3
Abstract
The development of nanomanipulation techniques has given investigators the ability to manipulate single biomolecules and to record mechanical events o f biomolecules at the single molecule level. The techniques were developed to elucidate the mechanism of molecular motors. We can directly monitor the unitary process of the mechanical work and the energy conversion processes by combining these techniques with the single molecule imaging techniques. Our results strongly suggest that the sliding movement of the actomyosin m otor is driven by Brownian movement. Other groups have reported data that a re more consistent with the lever arm model. These methods and imaging tech niques enable us to monitor the behavior of biomolecules at work and will h e applied to other molecular machines.