Suprarenal intraarterial infusion of alloxan and Streptozotocin during balloon occlusion of the juxtarenal abdominal aorta: A simple technique for inducing diabetes mellitus in canines with reduced mortality

Rationale and Objectives, The authors performed this study to evaluate the mortality and morbidity associated with a simple technique for inducing dia betes in dogs-suprarenal intraarterial infusion of alloxan and streptozotoc in during balloon occlusion of the juxtarenal abdominal aorta. Materials and Methods. The authors attempted to induce diabetes in six purp ose-bred dogs. After the dogs were fasted for 12 hours, the abdominal aorta at the level of the origin of the renal arteries was occluded with an angi oplasty balloon introduced by means of a femoral approach. A 3-F microcathe ter (n = 1) or infusion wire (n = 5) was introduced via the percutaneous tr ansluminal angioplasty catheter and positioned at the level of the celiac a xis, and a mixture of streptozotocin (20-25 mg/kg) and alloxan (20-25 mg/kg ) was infused. Diabetes was considered to have been induced if the dogs exp erienced sustained hyperglycemia. Results. There were no deaths during the follow-up period (range, 7 months to 21/2 years). A diabetes-like state was induced in five of the six dogs, and no nephrotoxicity was seen. Diabetes was not induced in one dog owing t o caudal migration of an undersized balloon during the infusion; this also resulted in reversible renal damage. Conclusion. This simple technique is effective for inducing diabetes in dog s, and morbidity and mortality rates are lower than those reported in the l iterature with other described techniques.