Dynamic study of the interaction between beta-lactoglobulin and phospholipids during complex film formation

Dynamic interfacial tension of phospholipid/protein adsorption was studied by ADSA(axisymmetric drop shape analysis, ADSA) to gain the information abo ut the mixed protein/phospholipid multilayers. AFM was used to study the mo rphology of the complex film. DPPE(dipalmitoyl phosphatidylethanolamine), D PPC (dipalmitoyl phosphatidylcholine), DPPA(dipalmitoyl phosphatidic acid) exhibited quite different adsorption behavior in the initial step due to th e net charge of their head group, but the interaction between phospholipid and protein seemed beyond the statistic force during the construction of pr otein/lipid multilayers when the different adsorption curves tended to coin cide at the end. The adsorption process was dominated by different kind int eractions at the different steps, respectively.