Evaluation of retinal damage induced by air/fluid exchange using a trypan blue inclusion test in rabbits

PURPOSE: To investigate whether retina damaged in the process of air/fluid exchange could be visualized in situ using trypan blue staining. METHODS: Vitrectomy and air/fluid exchange with continuous air infusion for various periods of time was performed in adult pigmented rabbits, followed by injection of 0.4% trypan blue solution into the vitreous cavity. Retina l staining was evaluated intraoperatively under the operating microscope an d by light microscopy after enucleation. RESULTS: Intraoperative examination showed localized trypan blue staining o f areas of the retina opposite the infusion cannula, Light microscopy of th e same areas revealed intranuclear trypan blue inclusion in the retina. CONCLUSION: Trypan blue staining allows for the immediate in situ evaluatio n of damage to the retina associated with air/fluid exchange and continuous air infusion in rabbit eyes. (Am J Ophthalmol 2001;131:814-815. (C) 2001 b y Elsevier Science Inc. All rights reserved.).