Ba. Kidney et al., Evaluation of formalin-fixed paraffin-embedded tissues from vaccine site-associated sarcomas of cats for polyomavirus DNA and antigen, AM J VET RE, 62(6), 2001, pp. 828-832
Objective-To determine whether vaccine site-associated sarcomas (VSS) from
cats contain polyomavirus antigen or DNA.
Sample Population-50 formalin-fixed paraffin-embedded tissue blocks of VSS
from cats.
Procedure-Sections from each tissue block were evaluated for polyomavirus a
ntigen by use of an avidin-biotin-complex immunohistochemical staining meth
od, using rabbit anti-murine polyomavirus polyclonal antiserum as the prima
ry antibody. The DNA was extracted from sections of each tissue block, and
a polymerase chain reaction assay was performed, using primers designed to
amplify regions of the bovine polyomavirus genome and consensus polyomaviru
s primers designed to detect unknown polyomaviruses.
Results-Polyomavirus antigen and DNA were not detected in any of the VSS.
Conclusions and Clinical Relevance-Results suggest that polyomaviruses like
ly do not have any direct involvement in the pathogenesis of VSS in cats.