Ba. Kidney et al., Evaluation of formalin-fixed paraffin-embedded tissues from vaccine site-associated sarcomas of cats for papillomavirus DNA and antigen, AM J VET RE, 62(6), 2001, pp. 833-839
Objective-To determine whether vaccine site-associated sarcomas (VSS) from
cats contain papillomavirus antigen or DNA.
Sample Population-50 formalin-fixed paraffin-embedded tissue blocks of VSS
from cats.
Procedure-Sections from each tissue block were evaluated for papillomavirus
antigen by use of an avidin-biotin-complex immunohistochemical staining me
thod, using rabbit anti-bovine papillomavirus type-1 antibody. The DNA was
extracted from sections of each tissue block, and polymerase chain reaction
assays were performed, using primers designed to amplify regions of the E5
gene of bovine papillomavirus and consensus primers designed to amplify a
region of the L1 gene of animal papillomaviruses. Sections from 20 of the t
issue blocks were evaluated by use of nonradioactive in situ hybridization
for bovine papillomavirus DNA.
Results-Papillomavirus antigen and DNA were not detected in any of the VSS.
Conclusions and Clinical Relevance-Results suggest that papillomaviruses li
kely do not have any direct involvement in the pathogenesis of VSS in cats.