On-line monitoring of nitric oxide complexed with porphyrine-bearing biochemical materials by using flow injection with chemiluminescence detection

An on-line nitric oxide (NO) monitoring method is described employing a flo w injection manifold with chemiluminescence (CL) detection. The method is t ailored for monitoring the NO from its complexes with porphyrin ring-bearin g (PRB) biochemical materials. The method is optimized for sensitivity and detection limit. The method is quite sensitive, simple, rapid, and precise. A sensitivity of 2.63 x 10(5) mV mol(-1) NO, a range of 2.0 x 10(-5) up to 1.0 x 10(-2) mol NO (under the conditions used) a reproducibility of 1.0% over the range 1.0 x 10(-3) up to 1.0 x 10-2 mol NO, a frequency of ca. fiv e samples per minute and a detection limit of 1.0 x 10(-5) mol NO are the a nalytical figures of merit for the proposed method. (C) 2001 Elsevier Scien ce B.V. All rights reserved.