The thermal and chemical stability of a theophylline imprinted methacrylic
acid-ethylene glycol dimethacrylate co-polymer has been investigated. Radio
ligand binding studies have been used to study the changes in theophylline
recognition characteristics of this and a non-imprinted, reference polymer
arising from exposure to elevated temperatures and a range of chemical envi
ronments. Polymers were shown to withstand exposure to temperatures of up t
o 150 degreesC (for 24 h) without loss of affinity for the template. Temper
atures above this point induced a rapid loss in binding capacity and a conc
omitant mass loss. FT-IR indicated that this arises from decarboxylation of
the polymer. The polymers exhibited remarkable resistance to extremes of p
H, organic base and to autoclave treatment. This study provides an indicati
on as to the limits of polymer stability, which is of significance for the
practical use of this type of polymer, e.g. in sensor-based applications. (
C) 2001 Elsevier Science B.V. All rights reserved.