A new concept for the construction of an artificial dehydrogenase for fructosylamine compounds and its application for an amperometric fructosylaminesensor

For the construction of an artificial dehydrogenase and an artificial dehyd rogenase based sensor, the potential application of polyvinylimidazole (PVI ) was investigated as the catalyst for the fructosylvaline (Fru-val) oxidat ion, a model compound for the glycated hemoglobin. The presence of PVI cata lyzed the oxidation of Fru-val in the presence of an electron acceptor. A c olorimetric determination of Fru-val was possible with the detection range from 50 muM to 10mM utilizing PVI as the catalyst. An amperometric sensor f or Fru-val was constructed using carbon paste electrode immobilized PVI. Us ing the sensor system, Fru-val could be measured from 20 muM to 0.7 mM. The se results indicated the further possible application of PVI as the catalys t for fructosylamine compounds as well as various glycated proteins includi ng glycated hemoglobin. (C) 2001 Elsevier Science B.V. All rights reserved.