Calcium inhibits human placental 11 beta-hydroxysteroid dehydrogenase type2 activity

The effect of Ca2+ on the conversion of cortisol to its inert metabolite co rtisone, the reaction catalyzed by the microsomal enzyme 11 beta -hydroxyst eroid dehydrogenase type 2 (11 beta -HSD2), was investigated in human place ntal microsomes. Placental microsomal 11 beta -HSD2 activity, as determined by the rate of conversion of cortisol to cortisone, was inhibited up to 50 % by increasing free Ca2+ concentrations from 22 to 268 nM. The Ca2+-induce d inhibition was reversible since chelation of endogenous Ca2+ with EGTA in creased 11 beta -HSD2 activity up to 200%. Ca2+ decreased the maximal veloc ity (V-max) of the 11 beta -HSD2 catalyzed conversion of cortisol to cortis one without altering the K-m of 11 beta -HSD2 for cortisol, indicating that Ca2+ modulates the catalytic efficiency rather than the substrate binding of 11 beta -HSD2. Moreover, the Ca2+-induced inhibition does not appear to involve altered cofactor (NAD(+)) binding since the inhibition of microsoma l 11 beta -HSD2 activity by a sub-maximal concentration of free Ca2+ was no t overcome by increasing the concentration of NAD(+). These findings in the microsomes were then extended to an intact cell system, JEG-3 cells, an es tablished model for human placental trophoblasts. In these cells, an increa se in cytosolic free Ca2+ concentration ([Ca2+](i)) elicited by a known phy siological stimulus, PGF(2 alpha), was accompanied by a 40% decrease in the level of 11 beta -HSD2 activity. Furthermore, the PGF(2 alpha)-induced inh ibition of 11 beta -HSD2 activity was abrogated when increases in [Ca2+](i) were blocked with the intracellular Ca2+ chelator, BAPTA. Collectively, th ese results demonstrate for the first time that Ca2+ inhibits human placent al 11 beta -HSD2 activity by a post-translational mechanism not involving s ubstrate or cofactor binding. (C) 2001 Academic Press.