Jh. Jeong et al., Characterization of the manganese-containing superoxide dismutase and its gene regulation in stress response of Schizosaccharomyces pombe, BIOC BIOP R, 283(4), 2001, pp. 908-914
Citations number
56
Categorie Soggetti
Biochemistry & Biophysics
Journal title
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Fission yeast Schizosaccharomyces pombe contains two superoxide dismutases
(SODs), one in the cytosol and the other in mitochondria. The sod2(+) gene
encoding putative mitochondrial superoxide dismutase containing manganese (
MnSOD) has been isolated. Purification and analysis of the sod2(+) gene pro
duct revealed that it contained only manganese as a cofactor, thus verified
to be a genuine MnSOD. It was localized in mitochondria as expected. Its N
-terminal amino acid sequence indicated that the mitochondrial targeting se
quence of 21 amino acids was removed. The native form consisted of two iden
tical subunits. The sod2(+) expression was induced by external stresses, su
ch as treatments with superoxide generators, high osmolarity, and heat. The
induction by these stress treatments depended on Wis1-Spc1 MAPK signal tra
nsduction pathway being independent of transcription factors Atf1 or Pap1.
The sod2 disruption rendered cells sensitive to various superoxide-generato
rs, heat, and high osmolarity, suggesting that the mitochondrial MnSOD acts
as a general defense agent against multiple stresses. (C) 2001 Academic Pr
ess.