Effect of sucrose concentration, charcoal, and indole-3-butyric acid on germination of Abies numidica somatic embryos

The dependency of radicle elongation in Abies numidica somatic embryos on g ermination media has been studied. No significant differences were detected between the Murashige and Skoog (MS) and Schenk and Hildebrandt (SH) mediu m. The addition of 10 g dm(-3) activated charcoal or 0.05 mg dm(-3) indole- 3-butyric acid (IBA) into both media had positive influence on embryo germi nation. Difference between activated charcoal and IBA effects were signific ant. The high rooting percentage (85 %) was recorded on half SH medium with 10 g dm(-3) sucrose and activated charcoal. After IBA addition rooting per centage was increased to 95 %. During 7 months 73 % of plantlets survived t ransfer to soil and in 54 % of plantlets shoot growth was observed.