SPECIFIC DETECTION OF CAMPYLOBACTER-LARI BY PCR

Citation
Oa. Oyarzabal et al., SPECIFIC DETECTION OF CAMPYLOBACTER-LARI BY PCR, Journal of microbiological methods, 29(2), 1997, pp. 97-102
Citations number
36
Categorie Soggetti
Microbiology,"Biochemical Research Methods
ISSN journal
01677012
Volume
29
Issue
2
Year of publication
1997
Pages
97 - 102
Database
ISI
SICI code
0167-7012(1997)29:2<97:SDOCBP>2.0.ZU;2-S
Abstract
Campylobacter lari is a bacterium associated infrequently with human e nteritis. Its differentiation from C. jejuni and C. coli relies on a f ew biochemical tests whose efficacy is highly questioned; therefore, t he incidence and transmission of this pathogen has not been well studi ed. Two novel oligonucleotide primers for the polymerase chain reactio n (PCR) that specifically amplify a 579-bp segment of the 16S rRNA gen e of C. lari under optimized conditions were designed. No PCR product was detected when DNA from other strains of Campylobacter, Arcobacter, Helicobacter, Escherichia, Salmonella or Listeria was used as templat es. Fast identification of C. lari through the present technique may a id the understanding of its incidence and epidemiology. (C) 1997 Elsev ier Science B.V.