This study has explored the localization and synthesis of the serglycin pro
teoglycan in the murine embryo and uterine decidua during midgestation. Emb
ryos in deciduae were subjected to in situ hybridization with cRNA probes a
nd to immunohistochemical detection with a specific antibody against murine
serglycin. Adherent decidual cell cultures were prepared from freshly isol
ated deciduae. Proteoglycan biosynthesis was investigated by labeling intac
t deciduae and decidual cultures with S-35-sulfate. Serglycin mRNA was dete
cted by in situ hybridization throughout the mesometrial portion and at the
periphery of the antimesometrial portion of the decidua at Embryonic Day (
E) 8.5, and in the parietal endoderm surrounding the embryo. Serglycin mRNA
was detected in fetal liver at E11.5-E14.5. Serglycin was detected by immu
nohistochemistry in decidua and parietal endoderm at E8.5 and in liver at E
13.5. Most of the proteoglycans synthesized by cultured intact deciduae (78
%) and adherent decidual cultures (91%) were secreted into the medium. Serg
lycin proteoglycan may play an important role in uterine decidual function
during early postimplantation development.