Ka. Vonnahme et al., Relationship between placental vascular endothelial growth factor expression and placental/endometrial vascularity in the pig, BIOL REPROD, 64(6), 2001, pp. 1821-1825
We investigated the temporal association between placental vascular endothe
lial growth factor (VEGF), a potent stimulator of angiogenesis and vascular
permeability, and changes in placental/endometrial vascularity on selected
days throughout gestation in the pig. Placental and endometrial tissues we
re collected from sows on Days 25 (n = 4), 36 (n = 6), 44 (n = 6), 70 (n =
5), 90 (n = 5), and 112 (n = 7) of gestation. Cross sections of the placent
al/endometrial interface of each conceptus were used to estimate the number
of blood vessels per unit area via image analysis and the intensity of VEG
F staining via immunohistochemistry. Placental tissues were also collected
on these days to evaluate VEGF mRNA expression. Placental VEGF mRNA express
ion and the numbers of blood vessels per unit area of placental and adjacen
t endometrial tissue were low and decreasing from Day 25 to Day 44, before
increasing (P < 0.05) markedly and progressively through Day 112. These dat
a are consistent with the marked increase in VEGF immunostaining in the cho
rionic and uterine luminal epithelium from early to late gestation. Further
, these increases in placental VEGF mRNA were positively correlated with fe
tal weight (r = 0.73; P < 0.0001) and placental efficiency (fetal weight/pl
acental weight ratio; r = 0.66, P < 0.0001). These data are consistent with
a role for VEGF in increasing the number of blood vessels at the placental
endometrial interface, resulting in an increased capacity for nutrient tra
nsfer from the maternal to the fetal compartment.