N. Elvassore et al., Production of different morphologies of biocompatible polymeric materials by supercritical CO2 antisolvent techniques, BIOTECH BIO, 73(6), 2001, pp. 449-457
High-value biocompatible-polymers have been processed with supercritical an
tisolvent techniques to produce solid structures of different shape and siz
e. In particular, a class of hyaluronic acid-derived polymers (Hyaff11-p100
, Hyaff11-p80, Hyaff11-p75, Hyaff 302) have been used to obtain various mor
phologies such as microspheres, threads, fibers, networks, and sponges. The
effect of thermodynamic variables on precipitation were highlighted in som
e preliminary batch experiments. Then, different products were obtained by
tuning the values of operating parameters. Threads and fibers were the resu
lt of a continuous supercritical antisolvent (SAS) process where a concentr
ated polymer solution was pumped through a micrometric nozzle: The threads
showed a reticular internal structure with an adjustable type of cavity. Fo
r production of networks and sponges, the concentration of polymer plays th
e key role. Below a critical value it was not possible to obtain a continuo
us network, while above it, a structure similar to that of the natural bone
with three types of internal microporosity were obtained. Again, by tuning
pressure and polymer concentration, the internal porosity could be control
led. Microparticles were also produced by the SAS process, and a control of
their morphology was achieved by varying the concentration of the polymer
in the starting solution and the density of organic solvent-CO2 mixtures. A
ll the products obtained by SAS have negligible content of residual solvent
. A qualitative interpretation of experimental results is presented. (C) 20
01 John Wiley & Sons, Inc.