Analysis of mono- and oligosaccharides by multiwavelength surface plasmon resonance (SPR) spectroscopy

Surface plasmon resonance (SPR) spectra of different saccharides were colle cted using a home-made multiwavelength SPR apparatus. Pentoses, hexoses, di saccharides and a trisaccharide were distinguished from one another accordi ng to their SPR spectra collected at the same concentration. The spectra we re also used for the quantitation of sugars by exploring the linear relatio nship between resonance wavelength and solute concentration. The dynamic li near ranges for the determination of glucose, sucrose and raffinose are 0.0 1-0.2, 0.005-0.1 and 0.0025-0.1 mol/L, respectively. The SPR spectrum of a mixture of two components was investigated. While the experiments have not been carried out, the implications from this work are that the technique wo uld be applicable to mixtures containing more than two components. (C) 2001 Elsevier Science Ltd. All rights reserved.